Mengarelli Isabella, Fryga Andrew, Barberi Tiziano
Department of Experimental Cardiology, Academic Medical Center, University of Amsterdam, K2-113, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands,
Methods Mol Biol. 2016;1307:191-203. doi: 10.1007/7651_2013_55.
Flow Cytometry-Sorting (FCM-Sorting) is a technique commonly used to identify and isolate specific types of cells from a heterogeneous population of live cells. Here we describe a multicolor flow cytometry technique that uses five distinct cell surface antigens to isolate four live populations with different surface antigen profiles. These profiles were used to help distinguishing between neural and nonneural (the lens) ectoderm derivatives within a highly heterogenous population of differentiating human embryonic stem cells (hESC).
流式细胞术分选（FCM-Sorting）是一种常用于从活细胞异质群体中识别和分离特定类型细胞的技术。在此，我们描述一种多色流式细胞术技术，该技术利用五种不同的细胞表面抗原分离出具有不同表面抗原谱的四个活细胞群体。这些谱被用于帮助区分高度异质的分化人胚胎干细胞（hESC）群体中的神经外胚层衍生物和非神经（晶状体）外胚层衍生物。
