College of Science, Ibaraki University, Mito, Japan.
FEBS J. 2014 Feb;281(4):1057-67. doi: 10.1111/febs.12668. Epub 2014 Jan 15.
It has been reported that minichromosome maintenance protein-binding protein (MCM-BP) functions in the formation of the pre-replication complex, unloading of minichromosome maintenance (MCM)2-7 from chromatin in late S phase, and formation of the cohesion complex by interacting with MCM3-7 proteins, suggesting that MCM-BP functions in several different reactions during the cell cycle. Here, we examined the interaction of human MCM-BP with MCM2-7 and structural maintenance of chromosome 3 in synchronized HeLa cells by immunoprecipitation. The results show that MCM-BP mainly interacts with MCM7 in the Triton-soluble fraction from S phase and G(2) phase cells, and it also interacts with structural maintenance of chromosome 3 in the fraction from G(2) phase cells. In vitro studies show that MCM-BP disassembles MCM2-7 bound to DNA with a fork-like structure by interacting with MCM3, MCM5, and MCM7. These results suggest that MCM-BP functions in disassembling MCM2-7 on chromatin during S phase and G2 phase by interacting with MCM3, MCM5, and MCM7.
据报道，微小染色体维持蛋白结合蛋白（MCM-BP）在复制前复合物的形成、S 期晚期从小染色体维持（MCM）2-7 从染色质上的卸载以及通过与 MCM3-7 蛋白相互作用形成黏合复合物中发挥作用，这表明 MCM-BP 在细胞周期的几个不同反应中发挥作用。在这里，我们通过免疫沉淀法检查了人 MCM-BP 与 MCM2-7 和染色体 3 结构维持蛋白在同步化 HeLa 细胞中的相互作用。结果表明，MCM-BP 主要与 S 期和 G2 期细胞的 Triton 可溶性部分中的 MCM7 相互作用，并且它还与 G2 期细胞的部分中的染色体 3 结构维持蛋白相互作用。体外研究表明，MCM-BP 通过与 MCM3、MCM5 和 MCM7 相互作用，将带有叉状结构的 DNA 结合的 MCM2-7 解组装。这些结果表明，MCM-BP 通过与 MCM3、MCM5 和 MCM7 相互作用，在 S 期和 G2 期在染色质上解组装 MCM2-7。
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