Said H M, Newsom A E, Feige A J, Lauren S L, Mathews R A
J Chromatogr. 1986 Aug 22;381(1):41-52. doi: 10.1016/s0378-4347(00)83563-x.
The human hair cystine-rich proteins have been separated through the combined use of reversed-phase and size-exclusion chromatography into more than fifty components. These have been grouped, based on molecular weight, into six families of closely related members. The families range in molecular weight from less than 6500 for the low-molecular-weight components to more than 67 000 for the high-molecular-weight components, with average intermediate values for the other families of 8000, 11 500, 15 500 and 19 000. The results also suggest an organized structure of the hair matrix proteins. The combined use of reversed-phase and size-exclusion high-performance liquid chromatography in these studies presents an example where the quaternary structure of a multi-component protein can be largely deduced from its chromatographic behaviour.
通过反相色谱法和尺寸排阻色谱法联用，已将富含胱氨酸的人发蛋白分离成五十多种成分。基于分子量，这些成分被分为六个由密切相关成员组成的家族。这些家族的分子量范围从低分子量成分的小于6500到高分子量成分的大于67000，其他家族的平均中间值分别为8000、11500、15500和19000。结果还表明毛发基质蛋白具有有序结构。在这些研究中反相和尺寸排阻高效液相色谱法的联用提供了一个实例，即多组分蛋白质的四级结构可从其色谱行为中大致推导出来。
