根据糖醛酸含量和单糖组成去除蓝藻胞外多糖中的金属。
Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition.
机构信息
Nevsehir University, Faculty of Science and Arts, Department of Molecular Biology and Genetics, Nevsehir, Turkey.
出版信息
Carbohydr Polym. 2014 Jan 30;101:265-71. doi: 10.1016/j.carbpol.2013.09.040. Epub 2013 Sep 21.
In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp. BASO671 were investigated. It was investigated that BASO671 showed different removal ability when exposed to each metal solely and mixed metal. EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II). Monomer composition of EPS was changed after metal treatment. Uronic acid contents of metal treated cells were higher than control cells of each isolate. Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells. Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface. Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
在本研究中，研究了 BASO671 对铬、镉和金属混合物（铬+镉）的去除及其与胞外多糖和糖醛酸生产的关系。研究表明，BASO671 在单独和混合金属暴露下表现出不同的去除能力。BASO671 在暴露于 15 和 35 ppm Cr(VI)、Cd(II)和 Cr(VI)+Cd(II)后，EPS 的产量增加。EPS 的单体组成在金属处理后发生了变化。金属处理细胞的糖醛酸含量高于每个分离株的对照细胞。此外，EPS 的葡萄糖醛酸含量和半乳糖醛酸含量与细胞的糖醛酸含量相关。扫描电子显微镜和能量色散 X 射线能谱分析证实，相当数量的金属已沉淀在细胞表面。EPS 的傅里叶变换红外光谱分析表明存在 CH 和 CO 基团，它们可能作为二价阳离子的结合位点。
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