Lenrstuhl für Botanik, Technische Universität München, Arcisstraße 21, D-8000, München 2, Federal Republic of Germany.
Planta. 1981 Jul;152(3):259-67. doi: 10.1007/BF00385154.
The fine structure of the upper cuticular membrane (CM) of Clivia miniata leaves was investigated using electron microscopy. The CM is made up of a thin (130 nm) lamellated cuticle proper (CP) and a thick (up to 7 μm over periclinal walls) cuticular layer (CL) of marbled appearance. Evidence is presented to show that the electron lucent lamellae of the CP do not simply represent layers of soluble cuticular lipids (SCL). Instead, the lamellation is probably due to layers of cutin differing in polarity. It is argued that the SCL in the Cp are the main barrier to water. Thickening of the CM during leaf development takes place by interposition of cutin between the CM and the cellin wall. The cutin of young, expanding leaves has a high affinity for KMnO4 and is therefore relatively polar. As leaves mature, the external CL underneath the CP becomes non-polar, as only little contrast can be obtained with permanganate as the post fixative.
使用电子显微镜研究了朱顶红叶片上表皮膜（CM）的精细结构。CM 由薄的（130nm）层状表皮固有层（CP）和厚的（沿垂周壁可达 7μm）大理石纹状的表皮层（CL）组成。有证据表明，CP 中的电子透明薄片并非简单地代表可溶性角质层脂质（SCL）的层。相反，层状结构可能是由于角质的极性不同所致。有人认为，CP 中的 SCL 是水分的主要屏障。在叶片发育过程中，CM 的增厚是通过角质层在 CM 和细胞壁之间的插入而发生的。在年轻、扩张的叶片中，角质对 KMnO4 具有高亲和力，因此相对极性。随着叶片的成熟，CP 下的外部 CL 变得非极性，因为用高锰酸盐作为后固定剂时只能获得很少的对比。
