Institut für Botanische Biologie der Universität, CH-1700, Freiburg, Switzerland.
Planta. 1984 Jan;161(1):1-11. doi: 10.1007/BF00951453.
Staining cuticular membranes ofAgave americana andClivia miniata en bloc with potassium permanganate results in a strong contrast in the interior cuticular layer while the exterior part remains unstained. This is not caused by a selective chemical reaction with the interior part but by the unidirectional penetration of the reagent from the interior side, the outside being protected by the cuticle proper. In transverse cryosections of the cuticular membrane, permanganate penetrates nearly as easily into the exterior cuticular layer as into the interior one giving the same contrast. However, compared with the periclinal penetration into the cuticle proper this penetration is accelerated five-to tenfold by the polysaccharide network within the cuticular layer which serves as a distribution-channel system. Periclinal penetration into the cuticle proper occurs independently in each "cutin penetration unit" included between two obvious lucent lamellae and further divided into subunits.
用高锰酸钾将龙舌兰和君子兰的表皮膜整体染色，会使内部表皮层形成强烈的对比，而外部部分保持不变。这不是由于与内部部分的选择性化学反应引起的，而是由于试剂从内部单向渗透引起的，外部受到表皮的保护。在表皮膜的横向冷冻切片中，高锰酸钾几乎可以同样容易地渗透到外部表皮层和内部表皮层，形成相同的对比。然而，与垂直渗透到表皮本身相比，这种渗透在角质层内的多糖网络的作用下被加速了五到十倍，多糖网络作为一个分配通道系统。垂直渗透到表皮本身是在每个“角质渗透单元”中独立发生的，每个“角质渗透单元”都夹在两个明显的透明薄片之间，并进一步分为亚单位。
