Yang Xing-Jian, Dang Zhi, Zhang Fang-Li, Lin Zhao-Ying, Zou Meng-Yao, Tao Xue-Qin, Lu Gui-Ning
School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
ScientificWorldJournal. 2013 Nov 5;2013:840376. doi: 10.1155/2013/840376. eCollection 2013.
This study described the development of a method based on Soxhlet extraction combining high performance liquid chromatography (Soxhlet-HPLC) for the accurate detection of BDE-209 in soils. The solvent effect of working standard solutions in HPLC was discussed. Results showed that 1:1 of methanol and acetone was the optimal condition which could totally dissolve the BDE-209 in environmental samples and avoid the decrease of the peak area and the peak deformation difference of BDE-209 in HPLC. The preliminary experiment was conducted on the configured grassland (1 μ g/g) to validate the method feasibility. The method produced reliable reproducibility, simulated soils (n = 4) RSD 1.0%, and was further verified by the analysis e-waste contaminated soils, RSD range 5.9-11.4%. The contamination level of BDE-209 in burning site was consistent with the previous study of Longtang town but lower than Guiyu town, and higher concentration of BDE-209 in paddy field mainly resulted from the long-standing disassembling area nearby. This accurate and fast method was successfully developed to extract and analyze BDE-209 in soil samples, showing its potential use for replacing GC to determinate BDE-209 in soil samples.
本研究描述了一种基于索氏提取结合高效液相色谱法（索氏提取-高效液相色谱法）的方法的开发，用于准确检测土壤中的BDE-209。讨论了高效液相色谱中工作标准溶液的溶剂效应。结果表明，甲醇与丙酮1:1的比例是最佳条件，该条件能使环境样品中的BDE-209完全溶解，并避免高效液相色谱中BDE-209峰面积减小和峰形变差。在配置的草地（1μg/g）上进行了初步实验，以验证该方法的可行性。该方法具有可靠的重现性，模拟土壤（n = 4）的相对标准偏差为1.0%，并通过对电子垃圾污染土壤的分析进一步验证，相对标准偏差范围为5.9-11.4%。焚烧场中BDE-209的污染水平与之前对龙塘镇的研究一致，但低于贵屿镇，稻田中较高浓度的BDE-209主要源于附近长期存在的拆解区域。成功开发了这种准确快速的方法来提取和分析土壤样品中的BDE-209，显示出其在替代气相色谱法测定土壤样品中BDE-209方面的潜在用途。
