Departments of Urology, 1Yokohama General Hospital, Yokohama, Japan Showa University Fujigaoka Hospital, Yokohama, japan.
Int J Urol. 1996 Jan;3(1 Suppl):S32-4. doi: 10.1111/j.1442-2042.1996.tb00079.x.
Actively dividing cells produce a number of proteins that may serve as useful antigenic markers in immunological studies of cellular proliferation and malignant potential. We investigated the expression of PCNA, p53 and c-a erbB-2 proteins in transitional cell carcinoma of human urinary bladder using an immunohistochemical method, and compared the results with the histologic grades and stages. Immunohistochemical studies on these proteins using monoclonal or polyclonal antibodies were performed with formalin-fixed paraffin sections of tumor tissue from 40 patients with bladder cancer. Generally, higher-grade and higher-stage tumors expressed PCNA and p53 and c-erbB-2 proteins with greater frequency than tumors of a lower grade or lower stage. These results suggest that the detection of each antigen might be useful in estimating the malignant potential of transitional cell carcinoma as an adjuvant study, because of its applicability to paraffin-embedded tissue sections and its simple, rapid technique.
活跃分裂的细胞产生许多蛋白质，这些蛋白质可能作为细胞增殖和恶性潜能的免疫学研究中的有用的抗原标记物。我们使用免疫组织化学方法研究了人膀胱移行细胞癌中 PCNA、p53 和 c-a erbB-2 蛋白的表达，并将结果与组织学分级和分期进行了比较。使用针对这些蛋白质的单克隆或多克隆抗体，对来自 40 例膀胱癌患者的肿瘤组织的福尔马林固定石蜡切片进行了免疫组织化学研究。一般来说，与低分级或低分期的肿瘤相比，高级别和高分期的肿瘤表达 PCNA 和 p53 以及 c-erbB-2 蛋白的频率更高。这些结果表明，由于其适用于石蜡包埋组织切片以及其简单、快速的技术，每种抗原的检测可能有助于估计移行细胞癌的恶性潜能，作为辅助研究。
