Benedini-Elias Priscila Cação Oliveira, Morgan Mariana Calvente, Cornachione Anabelle Silva, Martinez Edson Z, Mattiello-Sverzut Ana Claudia
Skeletal Muscle Structure and Function, Laboratory of Scientific Research, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor Apparatus, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Department of Social Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Acta Histochem. 2014 Apr;116(3):503-13. doi: 10.1016/j.acthis.2013.10.008. Epub 2013 Dec 2.
This study investigated how different types of remobilization after hind limb immobilization, eccentric exercise and passive static stretching, influenced the adaptive responses of muscles with similar function and fascicle size, but differing in their contractile characteristics. Female Wistar weanling rats (21 days old) were divided into 8 groups: immobilized for 10 days, maintaining the ankle in maximum plantar flexion; immobilized and submitted to eccentric training for 10 or 21 days on a declining treadmill for 40min; immobilized and submitted to passive stretching for 10 or 21 days for 40min by maintaining the ankle in maximum dorsiflexion; control of immobilized; and control of 10 or 21 days. The soleus and plantaris muscles were analyzed using fiber distribution, lesser diameter, capillary/fiber ratio, and morphology. Results showed that the immobilization reduced the diameter of all fiber types, caused changes in fiber distribution and decreased the number of transverse capillaries in both muscles. The recovery period of the soleus muscle is longer than that of the plantaris after detraining. Moreover, eccentric training induced greater hypertrophic and angiogenic responses than passive stretching, especially after 21 days of rehabilitation. Both techniques demonstrated positive effects for muscle rehabilitation with the eccentric exercise being more effective.
本研究调查了后肢固定后不同类型的恢复训练，即离心运动和被动静态拉伸，如何影响功能相似、肌束大小相同但收缩特性不同的肌肉的适应性反应。将雌性Wistar断奶大鼠（21日龄）分为8组：固定10天，使踝关节保持在最大跖屈位；固定并在递减式跑步机上进行10或21天的离心训练，每次40分钟；固定并通过使踝关节保持在最大背屈位进行10或21天的被动拉伸，每次40分钟；固定对照组；以及10天或21天的对照组。使用纤维分布、较小直径、毛细血管/纤维比和形态学对比目鱼肌和跖肌进行分析。结果表明，固定会减小所有纤维类型的直径，导致纤维分布发生变化，并减少两块肌肉中横向毛细血管的数量。停训后，比目鱼肌的恢复时间比跖肌长。此外，离心训练比被动拉伸诱导出更大的肥大和血管生成反应，尤其是在康复21天后。两种技术都对肌肉康复有积极作用，其中离心运动更有效。
