Kjaerup Rikke M, Dalgaard Tina S, Norup Liselotte R, Bergman Ingrid-Maria, Sørensen Poul, Juul-Madsen Helle R
Department of Animal Science, Aarhus University, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark.
Department of Animal Science, Aarhus University, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark.
Immunobiology. 2014 Apr;219(4):263-74. doi: 10.1016/j.imbio.2013.10.013. Epub 2013 Nov 8.
Mannose-binding lectin (MBL) plays a major role in the immune response as a soluble pattern-recognition receptor. MBL deficiency and susceptibility to different types of infections have been subject to extensive studies over the last decades. In humans and chickens, several studies have shown that MBL participates in the protection of hosts against virus infections. Infectious bronchitis (IB) is a highly contagious disease of economic importance in the poultry industry caused by the coronavirus infectious bronchitis virus (IBV). MBL has earlier been described to play a potential role in the pathogenesis of IBV infection and the production of IBV-specific antibodies, which may be exploited in optimising IBV vaccine strategies. The present study shows that MBL has the capability to bind to IBV in vitro. Chickens from two inbred lines (L10H and L10L) selected for high or low MBL serum concentrations, respectively, were vaccinated against IBV with or without the addition of the MBL ligands mannan, chitosan and fructooligosaccharide (FOS). The addition of MBL ligands to the IBV vaccine, especially FOS, enhanced the production of IBV-specific IgG antibody production in L10H chickens, but not L10L chickens after the second vaccination. The addition of FOS to the vaccine also increased the number of circulating CD4+ cells in L10H chickens compared to L10L chickens. The L10H chickens as well as the L10L chickens also showed an increased number of CD4-CD8α-γδ T-cells when an MBL ligand was added to the vaccine, most pronouncedly after the first vaccination. As MBL ligands co-administered with IBV vaccine induced differences between the two chicken lines, these results indirectly suggest that MBL is involved in the immune response to IBV vaccination. Furthermore, the higher antibody response in L10H chickens receiving vaccine and FOS makes FOS a potential adjuvant candidate in an IBV vaccine.
甘露糖结合凝集素（MBL）作为一种可溶性模式识别受体，在免疫反应中起主要作用。在过去几十年中，MBL缺乏与对不同类型感染的易感性一直是广泛研究的主题。在人类和鸡中，多项研究表明MBL参与宿主对病毒感染的保护。传染性支气管炎（IB）是由冠状病毒传染性支气管炎病毒（IBV）引起的、在禽类产业中具有重要经济意义的高度传染性疾病。此前已有研究表明MBL在IBV感染的发病机制及IBV特异性抗体的产生中发挥潜在作用，这可能有助于优化IBV疫苗策略。本研究表明MBL在体外具有结合IBV的能力。分别选择血清MBL浓度高或低的两个近交系鸡（L10H和L10L），用添加或不添加MBL配体甘露聚糖、壳聚糖和低聚果糖（FOS）的IBV疫苗进行接种。在第二次接种后，向IBV疫苗中添加MBL配体，尤其是FOS，可增强L10H鸡而非L10L鸡中IBV特异性IgG抗体的产生。与L10L鸡相比，向疫苗中添加FOS还增加了L10H鸡循环CD4+细胞的数量。当向疫苗中添加MBL配体时，L10H鸡和L10L鸡的CD4-CD8α-γδ T细胞数量也增加，在首次接种后最为明显。由于与IBV疫苗共同给药的MBL配体在两个鸡系之间诱导了差异，这些结果间接表明MBL参与了对IBV疫苗接种的免疫反应。此外，接受疫苗和FOS的L10H鸡中更高的抗体反应使FOS成为IBV疫苗中潜在的佐剂候选物。
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