Ottawa Research Station, Research Branch, Agriculture Canada, Ottawa, Ontario, Canada.
Theor Appl Genet. 1979 Mar;55(2):65-7. doi: 10.1007/BF00285191.
Culture of Brassica campestris anthers at 35°C for one or three days prior to culture at 25°C significantly stimulated the yield of microspore-derived embryos. More than 100 plants were regenerated from cultured embryos and haploids were identified amongst them. The haploid frequency was greater than 70% if all small-flowered sterile plants were considered to be haploid. The yield of microspore-derived plants in B. campestris is approaching the level where anther culture may be utilized as a practical breeding tool.
在 25°C 培养之前，将 Brassica campestris 花药在 35°C 下培养 1 或 3 天，可显著刺激小孢子胚状体的产量。从培养的胚状体中再生出了 100 多株植物，其中鉴定出了单倍体。如果将所有小花不育植株都视为单倍体，则单倍体频率大于 70%。在 Brassica campestris 中，小孢子胚状体的产量接近可以利用花药培养作为一种实用的育种工具的水平。
