不同玉米器官在无氧条件下的多肽合成模式。

Patterns of polypeptide synthesis in various maize organs under anaerobiosis.

机构信息

Department of Genetics, University of California, 94720, Berkeley, CA, USA.

出版信息

Planta. 1980 Nov;150(1):89-94. doi: 10.1007/BF00385619.

Abstract

The pattern of protein synthesis was compared in several organs of maize (Zea mays L.) under aerobic and anaerobic conditions. Protein synthesis was measured by [(35)S]methionine incorporation and analysis by two-dimensional native-SDS (sodium lauryl sulfate) polyacrylamide gel electrophoresis and fluorography. The aerobic protein-synthesis profiles were very different for root, endosperm, scutellum and anther wall. However, except for some characteristic qualitative and quantitative differences, the patterns of protein synthesis during anaerobiosis were remarkably similar for these diverse organs and also for mesocotyl and coleoptile. The proteins synthesized were the anaerobic polypeptides (ANPs) which have been previously described in anaerobic roots of seedlings. Leaves exhibited no detectable protein synthesis under anaerobic conditions, and died after a short anaerobic treatment. Evidence is presented that the ANPs are not a generalized response to stress. This indicates that the ANPs are synthesized as a specific response to anaerobic conditions such as flooding.

摘要

比较了几种玉米（Zea mays L.）器官在有氧和无氧条件下的蛋白质合成模式。通过[（35）S]甲硫氨酸掺入和二维天然-SDS（十二烷基硫酸钠）聚丙烯酰胺凝胶电泳和荧光分析来测量蛋白质合成。根、胚乳、盾片和花药壁的有氧蛋白合成图谱差异很大。然而，除了一些特征性的定性和定量差异外，这些不同器官以及中胚轴和 coleoptile 的无氧蛋白合成模式非常相似。合成的蛋白质是先前在幼苗的无氧根中描述的无氧多肽（ANPs）。叶片在无氧条件下几乎不进行蛋白质合成，经过短暂的无氧处理后会死亡。有证据表明，ANPs 不是对压力的普遍反应。这表明 ANPs 是作为对缺氧等特定条件的特定反应而合成的，例如洪水。