Sachs M M, Freeling M, Okimoto R
Cell. 1980 Jul;20(3):761-7. doi: 10.1016/0092-8674(80)90322-0.
Anaerobic treatment drastically alters the pattern of protein synthesized by maize primary roots. During the first hour of anaerobiosis, aerobic protein synthesis is halted and there is an increase in the synthesis of a class of polypeptides with approximate molecular weights of 33,000 daltons. During the second hour of anaerobic treatment, the synthesis of another small group of polypeptides is initated. This group, the anerboic polypeptides (ANPs), accounts for > 70% of total protein synthesis after 5 hr of anaerobiosis, and is synthesized in basically the same ratio until root death (approximately 70 hr). The alcohol dehydrogenase polypeptides are major ANPs. RNA isolated from roots treated anaerobically for at least 24 hr directs the translation of only the anaerobic polypeptides. However, RNA from roots treated anaerobically for only 5 hr directs translation of both anaerobic and aerobic polypeptides. Thus an early response to anaerobic treatment is the suppression of aerobic message translation. Although the anaerobic polypeptides share a formal similarity to heat-shock proteins in animals, it is probable that the anaerobic genes are an adaptation to flooding.
厌氧处理极大地改变了玉米初生根合成蛋白质的模式。在厌氧处理的第一个小时内，需氧蛋白质合成停止，一类分子量约为33,000道尔顿的多肽合成增加。在厌氧处理的第二个小时，另一小类多肽的合成开始。这一类多肽，即厌氧多肽（ANPs），在厌氧处理5小时后占总蛋白质合成的70%以上，并且在根死亡（约70小时）之前基本上以相同的比例合成。乙醇脱氢酶多肽是主要的厌氧多肽。从厌氧处理至少24小时的根中分离出的RNA仅指导厌氧多肽的翻译。然而，仅厌氧处理5小时的根中的RNA指导厌氧多肽和好氧多肽的翻译。因此，对厌氧处理的早期反应是需氧信息翻译的抑制。尽管厌氧多肽在形式上与动物中的热休克蛋白相似，但厌氧基因很可能是对水淹的一种适应。
