Department of Botany, University of Nottingham, University Park, Nottingham, Great Britain.
Theor Appl Genet. 1979 May;55(3-4):97-9. doi: 10.1007/BF00295433.
Somatic hybrid plants regenerated following the fusion of leaf mesophyll protoplasts of Petunia parodii with those isolated from a cell suspension of albino P. inflata. These two species exhibit a unilateral cross-incompatability with a pre-zygotic mode of reproductive isolation preventing hybridizations with P. inflata as the maternal parent. Selection of somatic hybrids relied on the fact that unfused or homokaryon protoplasts of P. parodii did not develop beyond the cell colony stage while those of the putative somatic hybrids and albino P. inflata parent produced callus. Green somatic hybrid calluses were readily identified against the white background of P. inflata following complementation to chlorophyll synthesis proficiency and continued growth in hybrid cells. Shoots, and ultimately flowering plants, were identified as somatic hybrids based on their floral morphology and colour, chromosome number and the fact that they segregated for parental characters. The frequency of somatic hybrid production was comparable to that previously established for two sexually compatible Petunia species.
叶肉原生质体融合再生体细胞杂种，所用亲本为矮牵牛 Parodii 与白化型 Inf lata 的悬浮细胞。这两个物种表现出单向的配子体不亲和性，其生殖隔离发生在合子前，因此阻止了矮牵牛 Parodii 作为母本的杂交。体细胞杂种的选择依赖于以下事实：未融合或同核原生质体的矮牵牛 Parodii 不会超过细胞集落阶段，而疑似体细胞杂种和白化型 Inf lata 亲本的原生质体则产生愈伤组织。杂种细胞中的叶绿素合成能力得到补充并持续生长后，绿色体细胞杂种愈伤组织在白化型 Inf lata 的白色背景下很容易被识别。根据花形态和颜色、染色体数目以及它们分离出亲本特征的事实，将茎和最终开花植物鉴定为体细胞杂种。体细胞杂种的产生频率与先前建立的两种有性亲和矮牵牛物种相当。
