Department of Chemistry, State University of New York, 11794-3400, Stony Brook, New York.
J Chem Ecol. 1986 Feb;12(2):323-33. doi: 10.1007/BF01020559.
Sensory hair proteins from antennae of males of the wild silk moth,Antheraea polyphemus (Lepidoptera, Saturniidae) were incubated with radiolabeled 6E,11Z-hexadecadienyl acetate in the presence of unlabeled pheromone analogs as competitive inhibitors. The two extracellular proteins of importance, a highly active sensillar esterase and an abundant 15,000 mol wt binding protein, interact to degrade labeled pheromone less efficiently in the presence of certain unsaturated acetate analogs of the natural pheromone.Enzymatic hydrolysis of the acetate (or diazoacetate) was also examined for three pheromone analogs: [11,12-(3)H2]-6E,11Z-hexadecadienyl diazoacetate, [11,12-(3)H2]-hexadecyl acetate, and [9,10-(3)H2]-9Z-tetradecenyl acetate. The former two are poor substrates at concentrations over four orders of magnitude. The 9Z-14:Ac, however, is the best alternative substrate for this in vitro pheromone metabolism system. Unlabeled 9Z-14: Ac is also the best competitive inhibitor of the hydrolysis of labeled 6E, 11Z-16: Ac. Whereas the tritiated natural pheromone shows a flat response (ca. 40% conversion) to increasing concentrations from 3 × 10(-9) to 3 × 10(-6) M, tritiated 9Z-14: Ac is degraded more rapidly at higher concentrations.
从野桑蚕雄蛾触角的感觉毛蛋白中，用放射性标记的 6E，11Z-十六碳二烯基乙酸酯孵育，同时作为竞争性抑制剂使用未标记的信息素类似物。两种重要的细胞外蛋白，一种高度活跃的感器酯酶和一种丰富的 15000 mol wt 结合蛋白，在存在某些天然信息素的不饱和乙酸类似物时，相互作用以降低标记信息素的降解效率。还检查了三种信息素类似物的乙酸盐（或重氮乙酸盐）的酶水解：[11,12-（3）H2]-6E，11Z-十六碳二烯基重氮乙酸酯，[11,12-（3）H2]-十六烷基乙酸酯和[9,10-（3）H2]-9Z-十四碳烯基乙酸酯。前两种在浓度超过四个数量级时是较差的底物。然而，9Z-14：Ac 是这种体外信息素代谢系统的最佳替代底物。未标记的 9Z-14：Ac 也是标记 6E，11Z-16：Ac 水解的最佳竞争性抑制剂。虽然氚标记的天然信息素对从 3×10（-9）到 3×10（-6）M 的浓度增加显示出平坦的响应（约 40%转化），但氚标记的 9Z-14：Ac 在较高浓度下更快地降解。
