Department of Entomology, New York State Agricultural Experiment Station, 14456, Geneva, New York.
J Chem Ecol. 1986 Feb;12(2):431-50. doi: 10.1007/BF01020565.
A technique for mass-labeling was developed to study sex pheromone biosynthesis in the red-banded leafroller moth,Argyrotaenia velutinana. With this technique, the pheromone components and all fatty acyl groups in the pheromone gland were analyzed for incorporation of label in the same analytic ran with gas chromatography-mass spectrometry, using chemical ionization and selected ion monitoring (GC-SIM-CI-MS). Sex pheromone glands were incubated with fatty acids or triacylglycerols labeled with at least three deuterium atoms or carbon-13 atoms. The results of these incubations support an interpretation in which hexadecanoate is chain shortened to tetradecanoate, which is desaturated to produce (E)- and (Z)-11-tetradecenoate precursors for the sex pheromone components (E)- and (Z)-11-tetradecen-1-yl acetate. Labeled (E)- and (Z)-11-tetradecenoyl groups in synthetic triacylglycerols were not incorporated into the sex pheromone components, perhaps indicating that this lipid class is not a donor of the immediate fatty acyl precursors in sex pheromone biosynthesis.
一种批量标记技术被开发出来，用于研究红带卷叶蛾 Argyrotaenia velutinana 性信息素的生物合成。使用这种技术，通过化学电离和选择离子监测（GC-SIM-CI-MS），用至少三个氘原子或碳-13 原子标记的脂肪酸或三酰基甘油，在相同的分析中分析性信息素腺中的性信息素成分和所有脂肪酰基。这些孵育的结果支持这样一种解释，即十六烷酸被缩短为十四烷酸，然后去饱和生成（E）-和（Z）-11-十四烯酸前体，用于性信息素成分（E）-和（Z）-11-十四烯-1-基乙酸酯。标记的（E）-和（Z）-11-十四烯酰基在合成三酰基甘油中没有掺入性信息素成分中，这也许表明这种脂质类不是性信息素生物合成中直接脂肪酸前体的供体。
