Andersen J F, Metcalf R L
Department of Entomology, University of Illinois, 61801, Urbana, Illinois.
J Chem Ecol. 1986 Mar;12(3):687-99. doi: 10.1007/BF01012102.
Fractionation of headspace volatiles from Cucurbita maxima blossoms by high-performance liquid chromatography resulted in the isolation of a single component which was highly active in an electroantennogram bioassay on Diabrotica undecimpunctata howardi antennae. This compound was identified as indole by gas chromatography-mass spectrometry. Field-trapping bioassays were conducted which indicated that indole is a potent attractant of the western corn rootworm,D. virgifera virgifera, and the striped cucumber beetle,Acalymma vittatum. The southern corn rootworm,D. u. howardi, did not respond, despite its strong EAG response. The sex ratio ofD. v. virgifera found in indole-baited traps varied seasonally. Males were trapped in abundance in late July and later September, 1983, while females were more abundant August and early September. The effectiveness of indole as aD. v. virgifera attractant also varied seasonally. A prolonged period of depressed trap catches occurred in early August 1983, during the silking and tasseling period of the corn in the field where trapping was carried out.
通过高效液相色谱法对南瓜花顶空挥发物进行分馏，分离出一种单一成分，该成分在对十一星黄瓜甲虫触角进行的触角电位生物测定中具有高活性。通过气相色谱 - 质谱联用鉴定该化合物为吲哚。进行了田间诱捕生物测定，结果表明吲哚是西部玉米根虫（D. virgifera virgifera）和条纹黄瓜甲虫（Acalymma vittatum）的有效引诱剂。南方玉米根虫（D. u. howardi）尽管有强烈的触角电位反应，但没有反应。在吲哚诱饵诱捕器中捕获的D. v. virgifera的性别比例随季节变化。1983年7月下旬和9月下旬捕获了大量雄性，而8月和9月初捕获的雌性更多。吲哚作为D. v. virgifera引诱剂的有效性也随季节变化。1983年8月初，在进行诱捕的田间玉米抽丝和抽雄期，诱捕器捕获量出现了一段长时间的下降。
