Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Chikusa, 464, Nagoya, Japan.
Plant Mol Biol. 1985 Nov;5(6):339-46. doi: 10.1007/BF00037554.
Five subunits (α-, β-, γ-, δ- and δ'-subunits) of the six α∼δ'-and ε-subunits) in the F1 portion (F1ATPase) of sweet potato (Ipomoea batatas) mitochondrial adenosine triphosphatase were isolated by an electrophoretic method. The δ- and δ'-subunits were not distinguishable immunologically but showed completely different tryptic peptide maps, indicating that they were different molecular species. In vitro protein synthesis with isolated sweet potato root mitochondria produced only the α-subunit when analyzed with anti-sweet potato F1ATPase antibody reacting with all the subunits except the ε-subunit. Sweet potato root poly(A)(+)RNA directed the synthesis of six polypeptides which were immunoprecipitated by the antibody: two of them immunologically related to the β-subunit and the others to the δ- and δ'-subunits. We conclude that the α-subunit of the F1ATPase is synthesized only in the mitochondria and the β-, δ- and δ'-subunits are in the cytoplasm.
五个亚基（α-、β-、γ-、δ-和δ'-亚基）和六个α∼δ'-和 ε-亚基）在甘薯（Ipomoea batatas）线粒体三磷酸腺苷酶的 F1 部分（F1ATPase）通过电泳方法分离。δ-和 δ'-亚基在免疫学上不可区分，但显示出完全不同的胰蛋白酶肽图谱，表明它们是不同的分子物种。用分离的甘薯根线粒体进行体外蛋白质合成，当用抗甘薯 F1ATPase 抗体分析时，只产生 α-亚基，该抗体与除 ε-亚基以外的所有亚基反应。甘薯根 poly(A)(+)RNA 指导合成六种被抗体免疫沉淀的多肽：其中两种在免疫学上与β-亚基有关，其他与δ-和 δ'-亚基有关。我们得出结论，F1ATPase 的 α-亚基仅在线粒体中合成，而β-、δ-和 δ'-亚基在细胞质中。
