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根瘤菌弗雷德里希氏菌株 USDA191 中 R-prime 缺失突变体的形成和共生基因的鉴定。

The formation of R-prime deletion mutants and the identification of the symbiotic genes in Rhizobium fredii strain USDA191.

机构信息

Dept. of Genetics, Iowa State University, 50011, Ames, IA, U.S.A..

出版信息

Plant Mol Biol. 1986 Jan;6(1):41-51. doi: 10.1007/BF00021305.

DOI:10.1007/BF00021305
PMID:24307153
Abstract

R-prime plasmids were formed between the plasmid of Rhizobium fredii strain USDA191 containing nodulation and nitrogen-fixation genes, pRjaUSDA191c, and pRL180, and RP1 derivative. R. fredii USDA191 contains four HindIII fragments that hybridize with an 8.7 kb EcoRI fragment that contains nodulation genes from R. meliloti. These four fragments are on pRjaUSDA191c and are 15.5 kb, 12.5 kb, 6.8 kb, and 5.2 kb in size. A series of R-primes generated in E. coli of pRjaUSDA191c were transferred into a Nod(-) Nif(-) derivative of strain USDA191 to determine which nodulation region is necessary for nodule formation. Transconjugants containing the 12.5 kb and the 6.8 kb HindIII fragments on segments of pRjaUSDA191c produced nodules on soybean plants. However, transconjugants containing the 12.5 kb HindIII fragment alone were unable to form nodules, suggesting that the 6.8 kb HindIII fragment or the 6.8 kb and the 12.5 kb HindIII fragments together were needed for nodule formation. The 6.8 kb HindIII fragment was subcloned into the vector pVK102 and transferred into transconjugants containing no sequences homologous to R. meliloti nodulation DNA or to transconjugants containing only the 12.5 kb HindIII fragment. Nodules were formed on soybeans only when both the 12.5 kb and the 6.8 kb HindIII fragments were present in R. frediistrain USDA191.

摘要

根瘤农杆菌 USDA191 菌株的质粒，含有结瘤和固氮基因 pRjaUSDA191c 和 pRL180，与 RP1 衍生物之间形成了 R-prime 质粒。根瘤农杆菌 USDA191 含有四个 HindIII 片段，与含有根瘤菌 meliloti 结瘤基因的 8.7kb EcoRI 片段杂交。这四个片段位于 pRjaUSDA191c 上，大小分别为 15.5kb、12.5kb、6.8kb 和 5.2kb。一系列在大肠杆菌中生成的 pRjaUSDA191c 的 R-prime 被转移到 USDA191 菌株的 Nod(-)Nif(-) 衍生物中，以确定哪个结瘤区对于形成根瘤是必要的。含有 pRjaUSDA191c 上的 12.5kb 和 6.8kb HindIII 片段的转导子在大豆植株上形成根瘤。然而，仅含有 12.5kb HindIII 片段的转导子不能形成根瘤，表明 6.8kb HindIII 片段或 6.8kb 和 12.5kb HindIII 片段一起对于根瘤的形成是必要的。6.8kb HindIII 片段被亚克隆到载体 pVK102 中，并转移到不含有与根瘤菌 meliloti 结瘤 DNA 同源序列的转导子或仅含有 12.5kb HindIII 片段的转导子中。只有当 12.5kb 和 6.8kb HindIII 片段都存在于根瘤农杆菌 USDA191 中时，大豆上才会形成根瘤。

相似文献

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本文引用的文献

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Fast-growing rhizobia isolated from root nodules of soybean.从大豆根瘤中分离出的快速生长型根瘤菌。
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Megaplasmids in the plant-associated bacteria Rhizobium meliloti and Pseudomonas solanacearum.植物相关细菌苜蓿中华根瘤菌和青枯雷尔氏菌中的巨型质粒。
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