Wang Yi, Suman Devi S, Bertrand Jacques, Dong Limin, Gaugler Randy
Center for Vector Biology, Rutgers University, New Brunswick, NJ, USA.
Pest Manag Sci. 2014 Aug;70(8):1299-304. doi: 10.1002/ps.3702. Epub 2014 Feb 17.
The Asian tiger mosquito, Aedes albopictus (Skuse), transmits important arboviral diseases and displaces native species. This peridomestic mosquito deposits eggs in natural and artificial containers. Container larval habitats tend to be cryptic and, therefore, difficult to reach by conventional insecticide treatments. We have developed an autodissemination station that transfers the insect growth regulator, pyriproxyfen, from the station to oviposition sites. Mosquitoes visiting the station become contaminated with an oil sticker followed by pyriproxyfen powder before exiting.
In a room (31 m(3) ) bioassay a single station consistently achieved 100% Ae. albopictus pupal mortality against 10 oviposition containers. In a greenhouse (200 m(3) ) assay with two stations and 12 oviposition cups, 91.7% of the cups had been contaminated as shown, and 57.1% pupal mortality was recorded. Pyriproxyfen transfer was also detected by visualizing mosquito 'tarsal prints' using a fluorescent dye.
The oil and pyriproxyfen powder dual-treatment station enhanced autodissemination efficacy by increasing toxicant attachment and retention on contaminated females. The autodissemination station offers a targeted, less environmentally damaging approach to manage cryptic container species.
白纹伊蚊（Skuse）传播重要的虫媒病毒疾病，并取代本地物种。这种家栖性蚊虫在天然和人工容器中产卵。容器幼虫栖息地往往很隐蔽，因此传统杀虫剂处理难以触及。我们开发了一种自动传播站，可将昆虫生长调节剂吡丙醚从站内转移到产卵地点。访问该站的蚊子在离开前会被油性贴纸沾染，随后沾染吡丙醚粉末。
在一个31立方米的房间生物测定中，单个站点针对10个产卵容器始终能实现100%的白纹伊蚊蛹死亡率。在一个200立方米的温室测定中，使用两个站点和12个产卵杯，如图所示，91.7%的杯子被沾染，记录到57.1%的蛹死亡率。通过使用荧光染料可视化蚊子的“跗节印记”，也检测到了吡丙醚的转移。
油性和吡丙醚粉末双重处理站通过增加有毒物质在受污染雌蚊上的附着和留存，提高了自动传播效果。自动传播站为管理隐蔽的容器物种提供了一种有针对性的、对环境危害较小的方法。
