单纯疱疹病毒1型微小RNA在静止和潜伏感染细胞培养模型中的表达
Expression of herpes simplex virus 1 microRNAs in cell culture models of quiescent and latent infection.
作者信息
Jurak Igor, Hackenberg Michael, Kim Ju Youn, Pesola Jean M, Everett Roger D, Preston Chris M, Wilson Angus C, Coen Donald M
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
出版信息
J Virol. 2014 Feb;88(4):2337-9. doi: 10.1128/JVI.03486-13. Epub 2013 Dec 4.
Abstract
To facilitate studies of herpes simplex virus 1 latency, cell culture models of quiescent or latent infection have been developed. Using deep sequencing, we analyzed the expression of viral microRNAs (miRNAs) in two models employing human fibroblasts and one using rat neurons. In all cases, the expression patterns differed from that in productively infected cells, with the rat neuron pattern most closely resembling that found in latently infected human or mouse ganglia in vivo.
摘要
为便于对单纯疱疹病毒1型潜伏进行研究，已开发出静止或潜伏感染的细胞培养模型。我们利用深度测序分析了两种用人成纤维细胞的模型以及一种用大鼠神经元的模型中病毒微小RNA（miRNA）的表达情况。在所有情况下，其表达模式均与产生性感染细胞中的不同，大鼠神经元的模式与体内潜伏感染的人或小鼠神经节中发现的模式最为相似。
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