Kozomara Ana, Griffiths-Jones Sam
Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7. doi: 10.1093/nar/gkq1027. Epub 2010 Oct 30.
miRBase is the primary online repository for all microRNA sequences and annotation. The current release (miRBase 16) contains over 15,000 microRNA gene loci in over 140 species, and over 17,000 distinct mature microRNA sequences. Deep-sequencing technologies have delivered a sharp rise in the rate of novel microRNA discovery. We have mapped reads from short RNA deep-sequencing experiments to microRNAs in miRBase and developed web interfaces to view these mappings. The user can view all read data associated with a given microRNA annotation, filter reads by experiment and count, and search for microRNAs by tissue- and stage-specific expression. These data can be used as a proxy for relative expression levels of microRNA sequences, provide detailed evidence for microRNA annotations and alternative isoforms of mature microRNAs, and allow us to revisit previous annotations. miRBase is available online at: http://www.mirbase.org/.
miRBase是所有微小RNA序列和注释的主要在线数据库。当前版本（miRBase 16）包含超过140个物种中的15000多个微小RNA基因位点，以及超过17000个不同的成熟微小RNA序列。深度测序技术使新微小RNA的发现率急剧上升。我们已将来自短RNA深度测序实验的读数映射到miRBase中的微小RNA，并开发了网络界面来查看这些映射。用户可以查看与给定微小RNA注释相关的所有读数数据，按实验和计数筛选读数，并按组织和阶段特异性表达搜索微小RNA。这些数据可作为微小RNA序列相对表达水平的替代指标，为微小RNA注释和成熟微小RNA的替代异构体提供详细证据，并使我们能够重新审视以前的注释。miRBase可在线访问：http://www.mirbase.org/ 。
