Harrison D J, MacDonald M K
Histopathology. 1986 Sep;10(9):945-52. doi: 10.1111/j.1365-2559.1986.tb02592.x.
A study was made of the cells forming the crescents in human crescentic glomerulonephritis. The investigation was performed using a panel of antibodies with immunoperoxidase techniques in formalin fixed, paraffin embedded renal biopsy material. Some of the cells of glomerular crescents were found to contain cytokeratin intermediate filaments, as did some of the cells of the normal parietal epithelium of Bowman's capsule. Leucocytes were also found in crescents, often in the outer part, and their presence was associated with a mantle of inflammatory cells around the glomerulus. The use of paraffin embedded rather than frozen tissue allowed better histological assessment than has been possible in previous studies. The glomerular crescents appeared to be primarily epithelial in origin, with leucocytes contributing to the overall inflammatory response.
对人类新月体性肾小球肾炎中形成新月体的细胞进行了一项研究。该研究采用一组抗体，运用免疫过氧化物酶技术，对福尔马林固定、石蜡包埋的肾活检材料进行检测。发现肾小球新月体的一些细胞含有细胞角蛋白中间丝，鲍曼囊正常壁层上皮的一些细胞也含有细胞角蛋白中间丝。在新月体中也发现了白细胞，通常位于其外部，并且它们的存在与肾小球周围的一层炎症细胞有关。使用石蜡包埋而非冷冻组织，相较于以往研究，能实现更好的组织学评估。肾小球新月体似乎主要起源于上皮细胞，白细胞则参与了整体的炎症反应。
