Sharma R K, Singh K
Z Parasitenkd. 1977 Dec 29;54(3):251-6. doi: 10.1007/BF00390116.
Biochemical studies on the two transaminases GOT and GPT of swine kidney worm Stephanurus dentatus have been made. GOT has been found much more active than GPT. Enzyme activities are based on the formation of oxaloacetate (GOT) or pyruvate (GPT) from aspartic acid and alanine respectively with oxoglutarate. A linear relationship is observed between the enzyme concentration and activity. GOT shows a maximum activity at pH 8.0 and Michaelis constant 9 X 10(-3) M for male and 2.9 X 10(-3) M for female. GPT has an optimum pH of 7.5 and a Michaelis constant 19 X 10(-3) M for male and 8 X 10(-3) M for female. The optimum temperature for both GOT and GPT was 60 degrees C.
对猪肾虫（有齿冠尾线虫）的两种转氨酶谷草转氨酶（GOT）和谷丙转氨酶（GPT）进行了生化研究。已发现GOT比GPT活性高得多。酶活性分别基于天冬氨酸和丙氨酸与α-酮戊二酸形成草酰乙酸（GOT）或丙酮酸（GPT）。观察到酶浓度与活性之间呈线性关系。GOT在pH 8.0时显示最大活性，雄性的米氏常数为9×10⁻³ M，雌性为2.9×10⁻³ M。GPT的最适pH为7.5，雄性的米氏常数为19×10⁻³ M，雌性为8×10⁻³ M。GOT和GPT的最适温度均为60℃。
