Györke Adriana, Pop Loredana, Cozma Vasile
Parasitology and Parasitic Diseases Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine Cluj Napoca, 3-5 Calea Mănăştur, 400372 Cluj-Napoca, Romania.
Parasite. 2013;20:50. doi: 10.1051/parasite/2013052. Epub 2013 Dec 6.
We conducted a survey in broiler farms from Romania to establish prevalence and distribution of Eimeria species using single PCR assay. We found Eimeria spp. in 21 (91%) out of 23 flocks, and in 11 (92%) out of 12 farms. Four species of Eimeria were identified: E. acervulina (21/23; 91%), E. tenella (14/23; 61%), E. maxima (5/23; 22%) and E. praecox (3/23; 13%). Infection with a single species (E. acervulina) was detected in 6 (26%) infected flocks originated from large farms. Mixed infections were found in 15 (65%) flocks and the most prevalent combination was E. acervulina + E. tenella (8/23; 35%). Four flocks (17%) harboured mixed infection with E. acervulina + E. tenella + E. maxima. E. acervulina was significantly more prevalent in flocks that received ionophores as anticoccidial feed additives.
我们在罗马尼亚的肉鸡养殖场进行了一项调查,采用单重聚合酶链反应(PCR)检测方法来确定艾美耳球虫种类的流行情况和分布。我们在23个鸡群中的21个(91%)以及12个养殖场中的11个(92%)发现了艾美耳球虫。鉴定出了四种艾美耳球虫:堆型艾美耳球虫(21/23;91%)、柔嫩艾美耳球虫(14/23;61%)、巨型艾美耳球虫(5/23;22%)和早熟艾美耳球虫(3/23;13%)。在来自大型养殖场的6个(26%)受感染鸡群中检测到单一物种(堆型艾美耳球虫)感染。在15个(65%)鸡群中发现了混合感染,最常见的组合是堆型艾美耳球虫 + 柔嫩艾美耳球虫(8/23;35%)。四个鸡群(17%)存在堆型艾美耳球虫 + 柔嫩艾美耳球虫 + 巨型艾美耳球虫的混合感染。堆型艾美耳球虫在使用离子载体作为抗球虫饲料添加剂的鸡群中显著更常见。