Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
Vet Parasitol. 2011 Jun 10;178(3-4):350-4. doi: 10.1016/j.vetpar.2011.01.009. Epub 2011 Jan 19.
A previously described multiplex PCR was evaluated for the identification and prevalence of Eimeria species in market-age commercial chicken flocks in Ontario. The multiplex PCR based on species-specific RAPD-SCAR markers was able to distinguish six available laboratory strains of Eimeria species (E. tenella, E. maxima, E. necatrix, E. mitis, E. acervulina, and E. brunetti) and E. tenella, E. maxima and E. acervulina in unknown field samples, including multiple infections in single reactions. No backyard (0/77) and 20/360 market-age commercial chickens were oocyst-positive using standard fecal flotation methods. PCR identified E. tenella alone (9/360, 2.5%), E. maxima alone (5/360, 1.38%), E. maxima plus E. tenella (5/360, 1.38%) and E. acervulina alone (1/360, 0.27%) in market-age commercial broilers. This is probably the first time the multiplex PCR has been evaluated in poultry establishments in Canada and illustrates the value of the tool in coccidiosis epidemiology on commercial farms.
先前描述的多重 PCR 用于鉴定和流行安大略省商业鸡群中的艾美耳球虫种类。基于种特异性 RAPD-SCAR 标记的多重 PCR 能够区分六种可用的实验室艾美耳球虫株（柔嫩艾美耳球虫、巨型艾美耳球虫、毒害艾美耳球虫、缓艾美耳球虫、堆形艾美耳球虫和布氏艾美耳球虫）和未知野外样本中的柔嫩艾美耳球虫、巨型艾美耳球虫和堆形艾美耳球虫，包括单个反应中的多重感染。使用标准粪便漂浮法，后院鸡（0/77）和 20/360 只商品代肉鸡未发现卵囊阳性。PCR 鉴定出商品代肉鸡中单独的柔嫩艾美耳球虫（9/360，2.5%）、单独的巨型艾美耳球虫（5/360，1.38%）、巨型艾美耳球虫和柔嫩艾美耳球虫混合感染（5/360，1.38%）和单独的堆形艾美耳球虫（1/360，0.27%）。这可能是首次在加拿大的家禽养殖场评估多重 PCR，并说明了该工具在商业农场球虫病流行病学中的价值。
