Alam J, Whitaker R A, Krogmann D W, Curtis S E
J Bacteriol. 1986 Dec;168(3):1265-71. doi: 10.1128/jb.168.3.1265-1271.1986.
The structural gene for ferredoxin I, petF, from the cyanobacterium Anabaena sp. strain PCC 7120 has been isolated from a recombinant lambda library. Mixtures of tetradecanucleotides and heptadecanucleotides, each containing all possible DNA sequences corresponding to two separate regions of the ferredoxin amino acid sequence, were synthesized and used as hybridization probes to identify a genomic clone containing the coding sequence for the petF gene. The sequence of the entire petF coding region and portions of the 3'- and 5'-flanking regions was determined. The DNA sequence of petF suggests that, in contrast to the nucleus-encoded plant protein, cyanobacterial apoferredoxin is not synthesized as a higher-molecular-weight precursor. The Anabaena petF gene is a single-copy gene. During growth on complete medium it was transcribed into a monocistronic mRNA species of approximately 500 bases that initiated 100 base pairs upstream from the petF coding region.
已从重组λ文库中分离出蓝藻鱼腥藻属Anabaena sp.菌株PCC 7120的铁氧化还原蛋白I的结构基因petF。合成了十四聚核苷酸和十七聚核苷酸混合物，每种混合物都包含与铁氧化还原蛋白氨基酸序列的两个不同区域相对应的所有可能DNA序列，并用作杂交探针来鉴定包含petF基因编码序列的基因组克隆。测定了整个petF编码区以及3'和5'侧翼区部分的序列。petF的DNA序列表明，与细胞核编码的植物蛋白不同，蓝藻脱辅基铁氧化还原蛋白不是作为高分子量前体合成的。鱼腥藻petF基因是单拷贝基因。在完全培养基上生长期间，它被转录成一种约500个碱基的单顺反子mRNA，该mRNA从petF编码区上游100个碱基对处起始。
