Biology Department, University of Puget Sound, 98416, Tacoma, WA, USA.
Photosynth Res. 1994 Jul;41(1):35-52. doi: 10.1007/BF02184144.
We report the presence of a diverse number ofChloroflexus-like organisms in intertidal marine and submerged hypersaline microbial mats using light, infrared fluorescence, and electron microscopy. The intertidal organisms appear morphologically very similar to thermophilicC. aurantiacus while the 2 hypersaline strains are larger and have a more complex ultrastructure composed of chlorosome-bearing internal membranes that appear to arise as invaginations of the cell membrane. By comparing spectroradiometry of microbial mat layers with microscopic observations, we have confirmed that theChloroflexus-like organisms are major constituents of the hypersaline microbial mat communities. In situ studies on mat layers dominated byChloroflexus-like organisms showed that sulfide-dependent photoautotrophic activity sustained by near infrared radiation prevailed. Autoradiographic analyses revealed that autotrophy was sustained in the filaments by 750 nm radiation. Three morphologically distinct strains are now maintained in mixed culture. One of these appears to be growing photoautotrophically.
我们利用光学显微镜、红外荧光显微镜和电子显微镜技术，在潮间带海洋和淹没式高盐微生物席中发现了大量类似绿屈挠菌的微生物。潮间带微生物在形态上与嗜热的 C. aurantiacus 非常相似，而 2 株高盐菌株较大，具有更复杂的超微结构，由带有菌光体的内细胞膜组成，这些细胞膜似乎是细胞膜内陷形成的。通过比较微生物席层的分光光度法与微观观察，我们已经证实类似绿屈挠菌的微生物是高盐微生物席群落的主要组成部分。对以类似绿屈挠菌为主的席层进行的现场研究表明，近红外辐射支持的依赖硫化物的光自养活性占主导地位。放射自显影分析表明，750nm 辐射维持着丝状菌的自养生长。现在已经维持了 3 种形态明显不同的混合培养物。其中一种似乎正在进行光自养生长。
