Section de Biophysique des Protéines et des Membranes, DBCM/CEA et URA 1290 CNRS, C.E. Saclay, 91191, Gif/Yvette Cedex, France.
Photosynth Res. 1994 Jul;41(1):175-80. doi: 10.1007/BF02184158.
Resonance Raman experiments were performed on different green bacteria. With blue excitation, i.e. under Soret resonance or preresonance conditions, resonance Raman contributions were essentially arising from the chlorosome pigments. By comparing these spectra and those of isolated chlorosomes, it is possible to evaluate how the latter retain their native structure during the isolation procedures. The structure of bacteriochlorophyll oligomers in chlorosomes was interspecifically compared, in bacteriochlorophyllc- and bacteriochlorophylle- synthesising bacteria. It appears that interactions assumed by the 9-keto carbonyl group are identical inChlorobium limicola, Chlorobium tepidum, andChlorobium phaeobacteroides. In the latter strain, the 3-formyl carbonyl group of bacteriochlorophylle is kept free from intermolecular interactions. By contrast, resonance Raman spectra unambiguously indicate that the structure of bacteriochlorophyll oligomers is slightly different in chlorosomes fromChloroflexus auranticus, either isolated or in the whole bacteria.
我们对不同的绿菌进行了共振拉曼实验。用蓝光激发，即在 Soret 共振或预共振条件下，共振拉曼贡献主要来自于类菌胞体色素。通过比较这些光谱和分离的类菌胞体的光谱，可以评估后者在分离过程中如何保持其天然结构。我们比较了在合成细菌叶绿素 c 和细菌叶绿素 e 的绿菌中类菌胞体中细菌叶绿素低聚物的结构。结果表明，Chlorobium limicola、Chlorobium tepidum 和 Chlorobium phaeobacteroides 中 9-酮羰基的相互作用是相同的。在后一种菌株中，细菌叶绿素 e 的 3-甲酰羰基基团不受分子间相互作用的影响。相比之下，共振拉曼光谱明确表明，来自 Chloroflexus auranticus 的类菌胞体中的细菌叶绿素低聚物的结构与分离的或整个细菌中的类菌胞体略有不同。
