Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University, 980, Sendai, Japan.
Photosynth Res. 1994 Jul;41(1):211-23. doi: 10.1007/BF02184162.
Cross polarization/magic angle spinning (CP/MAS)(13)C (solid state high resolution) NMR spectra were observed for chlorosomes and BChlc aggregates. Similarity of both kinds of spectra (except for some signals assignable to proteins and lipids in chlorosomes) indicates that BChlc's in chlorosomes are present just as in synthetic BChlc aggregates. Chemical shifts for C13(1) carbonyl and C3(1) hydroxylethyl carbons indicate hydrogen bonding between them. Comparison of solution and solid state(13)C NMR chemical shifts shows the five coordinated nature of BChlc aggregates. Some chemical shift differences were attributable to ring currents shifts. Their comparisons with calculated ring current shift values predicted structures for the aggregates. Cross polarization dynamics of the CP/MAS(13)C NMR signals explored dynamic and structural nature of the BChlc aggregates.
我们观察了类囊体和 BChlc 聚集体的交叉极化/魔角旋转（CP/MAS）(13)C（固态高分辨率）NMR 谱。两种光谱（除了可归因于类囊体中蛋白质和脂质的一些信号外）的相似性表明，类囊体中的 BChlc 以与合成 BChlc 聚集体中相同的方式存在。C13(1) 羰基和 C3(1) 羟乙基碳原子的化学位移表明它们之间存在氢键。比较溶液和固态(13)C NMR 化学位移表明 BChlc 聚集体的五配位性质。一些化学位移差异归因于环电流位移。将它们与计算出的环电流位移值进行比较，预测了聚集体的结构。CP/MAS(13)C NMR 信号的交叉极化动力学探索了 BChlc 聚集体的动态和结构性质。
