Costa P P, Jacobsson B, Collins V P, Biberfeld P
J Histochem Cytochem. 1986 Dec;34(12):1683-5. doi: 10.1177/34.12.2431032.
Rehydrated paraffin sections of formalin-fixed, amyloid-containing tissues were treated with denaturing agents (guanidine and urea) and reducing agents (DDT and mercaptoethanol) before immunostaining, in an attempt to expose antigenic determinants hidden in the rigid structure of amyloid fibrils. Pre-treatment overnight with 6 M guanidine or urea was found beneficial, especially in specimens from familial amyloid polyneuropathy of the Portuguese type. Addition of reducing agents had no major effect. Modifications of this method may be useful in unmasking other antigens that are polymerized or considered destroyed by fixation and paraffin embedding.
对经福尔马林固定、含有淀粉样蛋白的组织进行水化后的石蜡切片，在免疫染色前先用变性剂（胍和尿素）和还原剂（二硫苏糖醇和巯基乙醇）处理，试图暴露隐藏在淀粉样纤维刚性结构中的抗原决定簇。发现用6M胍或尿素预处理过夜是有益的，特别是对于来自葡萄牙型家族性淀粉样多神经病的标本。添加还原剂没有显著效果。这种方法的改进可能有助于揭示其他因固定和石蜡包埋而聚合或被认为已被破坏的抗原。
