Department of Chemistry, Simon Fraser University, V5A 1S6, Burnaby, British Columbia, Canada.
J Chem Ecol. 1985 Aug;11(8):1053-70. doi: 10.1007/BF01020675.
Aggregation pheromones were isolated fromCryptolestes pusillus (Schönherr), a coleopteran pest of stored products. Porapak Q-captured beetle and frass volatiles were fractionated by preparative gas-liquid chromatography. The fractions were bioassayed with an arena olfactometer and/or with a two-choice, pitfall olfactometer. Three biologically active, male-produced compounds eliciting aggregation behavior from adultC. pusillus were isolated and identified by spectroscopic methods as (Z)-3-dodecenolide (I), (Z)-5-tetradecen-13-olide (II), and (Z,Z)-3,6-dodecadienolide (III). Compound I was the major volatile produced and was active alone. Compound II was not active alone, but synergized the response to I. Compound III was active alone at higher concentrations, but did not significantly increase the response when added to the most active mixture of I and II, and so it is probably not part of the aggregation pheromone. Pheromone production increased dramatically when the insects were aerated on a food source.
聚集信息素从Cryptolestes pusillus（Schönherr）中分离出来，Cryptolestes pusillus 是一种鞘翅目害虫，危害储藏的产品。Porapak Q 捕获的甲虫和粪便挥发物通过制备性气相色谱进行分离。用竞技场嗅觉计和/或双选择、陷阱嗅觉计对这些馏分进行生物测定。通过光谱方法鉴定出三种具有生物活性的、雄性产生的化合物，可引起成年 C. pusillus 的聚集行为，它们分别是（Z）-3-十二烯内酯（I）、（Z）-5-十四烯-13-内酯（II）和（Z，Z）-3,6-十二碳二烯内酯（III）。化合物 I 是产生的主要挥发物，具有单独的活性。化合物 II 单独不具有活性，但对 I 的反应有增效作用。化合物 III 在较高浓度下具有活性，但当添加到最有效的 I 和 II 混合物中时，并没有显著增加反应，因此它可能不是聚集信息素的一部分。当昆虫在食物源上通气时，信息素的产生会显著增加。
