Department of Animal Ecology, Ecology Building, Lund University, S-223 62, Lund, Sweden.
J Chem Ecol. 1985 Sep;11(9):1181-96. doi: 10.1007/BF01024107.
Female turnip moths (Agrotis segetum) from a laboratory culture inbred for more than 30 generations, and the offspring (first and third generation) from field-collected insects were analyzed individually for acetates and alcohols in the pheromone gland. Quantitative analysis of individual components was performed at the subnanogram level by gas chromatographymass spectrometry (selected ion monitoring). The titer of the pheromone, i.e., the sum of the homologous acetates (Z)-5-decenyl acetate, (Z)-7-dodecenyl acetate, and (Z)-9-tetradecenyl acetate was 2.0 ± 0.3 ng in the laboratory culture and 3.2 ± 0.6 ng in the "wild strain." There was no correlation between pheromone titer and female weight. The relative proportion of the pheromone components varied substantially between individuals, but there was no statistically significant difference between the two populations. The percentages of the respective compounds (¯X ± coefficient of variation) were 14.8 ± 127% for Z5-10:OAc, 55.6 ± 32% for Z7-12:OAc, and 29.6 ± 59% forZ9-14:OAc. The pheromone composition varied more in the wild strain than in the laboratory culture. The significance of the pheromone variation to the attraction of males was tested in a field experiment. The ratio of males trapped by the most attractive blend versus the least attractive one was 2.2.
从经过超过 30 代自交的实验室培养的雌性萝卜银纹夜蛾（Agrotis segetum），以及从野外采集的昆虫的后代（第一代和第三代）的信息素腺体中分别分析乙酸酯和醇。通过气相色谱-质谱（选择离子监测）在亚纳克级水平上对各个成分进行定量分析。信息素的效价，即同源乙酸酯（Z）-5-癸烯乙酸酯、（Z）-7-十二烯乙酸酯和（Z）-9-十四烯乙酸酯的总和，在实验室培养中为 2.0 ± 0.3 ng，在“野生株”中为 3.2 ± 0.6 ng。信息素效价与雌虫体重之间没有相关性。个体之间信息素成分的相对比例变化很大，但两个种群之间没有统计学上的显著差异。各个化合物的百分比（¯X ± 变异系数）分别为 Z5-10:OAc 为 14.8 ± 127%，Z7-12:OAc 为 55.6 ± 32%，Z9-14:OAc 为 29.6 ± 59%。野生株中的信息素组成比实验室培养中的变化更大。在野外实验中测试了信息素变化对雄性吸引力的意义。最具吸引力的混合物捕获的雄性与最不具吸引力的混合物捕获的雄性的比例为 2.2。
