Institut für Biologie II der, Universität Freiburg, Schänzlestr. 1, D-7800, Freiburg, F.R.G..
Plant Mol Biol. 1984 Jul;3(4):243-8. doi: 10.1007/BF00029660.
An approach to assess the extent of developmental gene expression of various regions of plastid (pt)DNA in mustard (Sinapis alba L.) is described. It involves cloning of most ptDNA regions. The cloned regions then serve as hybridization probes to detect and assess the abundance of complementary RNA sequences represented in total plastid RNA. By comparison of the hybridization pattern observed with plastid RNA from either dark-grown or light-grown plants it was found that many ptDNA regions are constitutively expressed, while several 'inducible' regions account for much higher transcript levels in the chloroplast than in the etioplast stage. The reverse situation, i.e. 'repressed' regions which would account for higher transcript levels in the etioplast, was not observed. The hybridization results obtained with RNA from 'intermediatetype' plastids suggest that transient gene expression is a common feature during light-induced chloroplast development. The time-course of gene expression differs for various ptDNA regions.
描述了一种评估芥菜（Sinapis alba L.）质体（pt）DNA 各个区域发育基因表达程度的方法。它涉及克隆大多数 ptDNA 区域。然后，克隆的区域作为杂交探针，用于检测和评估总质体 RNA 中互补 RNA 序列的丰度。通过比较用来自黑暗生长或光照生长的植物的质体 RNA 观察到的杂交模式，发现许多 ptDNA 区域是组成型表达的，而几个“诱导型”区域在叶绿体中的转录水平比在黄化质体阶段高得多。没有观察到相反的情况，即“受抑制”的区域在黄化质体中会有更高的转录水平。用“中间型”质体的 RNA 获得的杂交结果表明，光诱导的叶绿体发育过程中普遍存在瞬时基因表达。不同的 ptDNA 区域的基因表达时间进程不同。
