燕麦种子 mRNA 的特性：球蛋白 mRNA 众多的证据。

Characterization of developing oat seed mRNA: evidence for many globulin mRNAs.

机构信息

Department of Biochemistry, School of Medicine, University of Ottawa, K1N9B4, Ottawa, Ontario, Canada.

出版信息

Plant Mol Biol. 1985 Jul;4(4):205-10. doi: 10.1007/BF02418237.

Abstract

Polyadenylated mRNA from developing oat (Avena sativa L.) seeds was isolated and analyzed. Prominent mRNA species of 18S, 15S and 12S were observed; the 18S mRNA was judged to be esentially free of ribosomal RNA by hybridization analysis. Size fractionation andin vitro translation of this mRNA was performed. SDS, IEF-SDS gel electrophoresis and immunoprecipitation were used to analyze the translation products. It is shown that globulin mRNA (18S) accounts for roughly 30% of the total mRNA in developing seeds, the 12S and 15S mRNAs accounting for the remainder. The 18S mRNA directs the synthesis of a series of distinct but related polypeptides, suggesting that some of the heterogeneity seen in the oat globulins is at the amino acid sequence level.

摘要

从发育中的燕麦（Avena sativa L.）种子中分离并分析了多聚腺苷酸化的 mRNA。观察到明显的 18S、15S 和 12S mRNA 种类；通过杂交分析判断 18S mRNA 基本上不含核糖体 RNA。对该 mRNA 进行了大小分级和体外翻译。SDS、IEF-SDS 凝胶电泳和免疫沉淀用于分析翻译产物。结果表明球蛋白 mRNA（18S）约占发育中种子总 mRNA 的 30%，12S 和 15S mRNA 占其余部分。18S mRNA 指导一系列不同但相关的多肽的合成，表明在燕麦球蛋白中观察到的一些异质性是在氨基酸序列水平上的。