Biochemical Laboratory, Free University, de Boelelaan 1083, 1081 HV, Amsteram, The Netherlands.
Plant Mol Biol. 1985 Jul;4(4):219-24. doi: 10.1007/BF02418239.
Mitochondrial DNA ofSpirodela oligorhiza (duck weed) was analyzed with restriction enzymes. The genome size appears to be at least 250 kbp. Four different PstI fragments were cloned. These four clones contain a sequence which is reiterated about 100-fold on theSpirodela mitochondrial DNA. Hybridization analysis showed that a similar sequence is present onZea mays mitochondrial DNA, although much less reiterated here. The presence of these reiterated sequences might contribute to the physical heterogeneity of plant mitochondrial DNA.
采用限制酶对浮萍(满江红)的线粒体 DNA 进行了分析。基因组大小似乎至少为 250 kbp。克隆了四个不同的 PstI 片段。这四个克隆包含一个序列,在浮萍线粒体 DNA 上重复约 100 倍。杂交分析表明,玉米线粒体 DNA 中存在类似的序列,尽管此处重复次数要少得多。这些重复序列的存在可能有助于植物线粒体 DNA 的物理异质性。