CSIRO Division of Horticultural Research, Box 350 GPO, 5001, Adelaide, South Australia.
Plant Mol Biol. 1985 Sep;4(5):267-73. doi: 10.1007/BF02418245.
A PstI 7.7 kbp fragment from chloroplast (ct) DNA of spinach shows homology to an EcoRI 8.3 kbp fragment of mitochondrial (mt) DNA and in turn, both are homologous to a number of common regions of nuclear (n) DNA. The common area of homology between the chloroplast and mitochondrial fragments is between a KpnI 1.8 segment internal to the PstI sites in the ctDNA and an EcoRI/BamHI 2.9 kbp fragment at one end of the mitochondrial 8.3 kbp fragment. The KpnI 1.8 kbp ctDNA fragment is within a structural gene for the P700 chlorophyll a apoprotein. Further analysis of this KpnI 1.8 kbp fragment confined the homologous region in mtDNA to a ct 0.8 kbp HpaII fragment. These smaller pieces of the organellar genomes share homologies with nuclear DNA as well as displaying unique hybridization sites. The observations reported here demonstrate that there is a common or closely related sequence in all three genetic compartments of the cell.
菠菜叶绿体（ct）DNA 的 PstI7.7kbp 片段与线粒体（mt）DNA 的 EcoRI8.3kbp 片段具有同源性，而这两个片段又与细胞核（n）DNA 的许多共同区域具有同源性。叶绿体和线粒体片段之间的共同同源区位于 ctDNA 中 PstI 位点内部的 KpnI1.8 片段和线粒体 8.3kbp 片段一端的 EcoRI/BamHI2.9kbp 片段之间。KpnI1.8kbpctDNA 片段位于 P700 叶绿素 a 脱辅基蛋白的结构基因内。对该 KpnI1.8kbp 片段的进一步分析将 mtDNA 中的同源区限定在 ct0.8kbpHpaII 片段内。这些细胞器基因组的较小片段与核 DNA 具有同源性，并显示出独特的杂交位点。这里报道的观察结果表明，细胞的所有三个遗传隔室中都存在共同或密切相关的序列。
