Department of Biology, University of Ottawa, K1N 6N5, Ottawa, Ontario, Canada.
Plant Mol Biol. 1985 Sep;4(5):285-92. doi: 10.1007/BF02418247.
Leghemoglobin is a major component of the nitrogen-fixing nodules formed by legumes in association with bacterial symbionts of the genusRhizobium. It is thought to be involved in regulating the oxygen tension within nodules. In a series of Southern blot experiments using cloned soybean leghemoglobin cDNAs as hybridization probes, cross-hybridizing sequences have been detected in legumes closely related to soybean (members of the Leguminosae subfamily Papilionoideae), as well as in a distantly related legume not reported to be nodulated (subfamily Caesalpinioideae). With the same probes, the presence of cross-hybridizing sequences has also been detected in plants outside the Leguminosae, including two nitrogen-fixing non-legumes and one species which is not nodulated. These results suggest that the genes for oxygen-binding proteins may be more widely dispersed than previously thought.
豆血红蛋白是豆科植物与根瘤菌属细菌共生形成的固氮结瘤的主要成分。它被认为参与调节结瘤内的氧气张力。在一系列使用克隆的大豆豆血红蛋白 cDNA 作为杂交探针的 Southern 印迹实验中，在与大豆密切相关的豆科植物（蝶形花亚科成员）中检测到了交叉杂交序列，以及在远缘豆科植物（苏木亚科）中未报道结瘤的植物中也检测到了交叉杂交序列。使用相同的探针，在豆科植物之外的植物中也检测到了交叉杂交序列，包括两种固氮非豆科植物和一种不结瘤的植物。这些结果表明，与氧气结合蛋白的基因可能比以前认为的更为广泛地分散。
