Department of Biological Sciences, Wayne State University, 48202, Detroit, MI, USA.
Plant Mol Biol. 1985 Sep;4(5):327-9. doi: 10.1007/BF02418254.
The RNA genomes of some plant viruses have properties which make them suitable for development into vectors for gene transfer. It has been claimed that the low fidelity of RNA synthesizing enzymes may limit the usefulness of such vectors. Arguments are presented here to indicate that the problem may not be serious. Examples are presented of plant RNA replicons which carry relatively stable coding sequences whose products are superfluous to either the replicon or the host. In addition, there are numerous instances in which slowly replicating virus strains are maintained in 'pure' culture in a host and are not overgrown by more rapidly replicating strains. It is difficult to estimate the error frequency of RNA replicating enzymes. Methods which rely on host-shift may be subject to error.
一些植物病毒的 RNA 基因组具有使其适合发展成为基因转移载体的特性。有人声称，RNA 合成酶的低保真度可能会限制此类载体的用途。本文提出的论点表明，该问题可能并不严重。本文还介绍了一些植物 RNA 复制子，它们携带相对稳定的编码序列，这些产物对复制子或宿主都是多余的。此外，还有许多情况下，在宿主中以“纯”培养的方式维持复制缓慢的病毒株，而不会被复制更快的株系所超过。很难估计 RNA 复制酶的错误频率。依赖于宿主转移的方法可能会存在误差。
