硫化氢和半胱氨酸调节菜豆初生叶片中腺苷 5′-磷酸硫酸酯磺基转移酶的活性
Regulation of adenosine 5'-phosphosulfate sulfotransferase activity by H2S and cyst(e)ine in primary leaves of Phaseolus vulgaris L.
机构信息
Pflanzenphysiologisches Institut der Universität Bern, Altenbergrain 21, CH-3013, Bern, Switzerland.
出版信息
Planta. 1979 Oct;147(1):37-42. doi: 10.1007/BF00384588.
During chloroplast development in the primary leaves of Phaseolus vulgaris, the extractable activity of adenosine 5'-phosphosulfate sulfotransferase increased ten-fold. When chloroplast development took place in air enriched with 3.5 μl H2S·l(-1) there was a decrease in adenosine 5'-phosphosulfate sulfotransferase activity. Cyst(e)ine in concentrations up to 1 mM (in the external medium) did not affect the increase in adenosine 5'-phosphosulfate sulfotransferase activity in intact plants. In plants with excised roots, 0.75 mM cyst(e)ine inhibited this increase. In green primary leaves, H2S or cyst(e)ine treatment resulted in a decrease of extractable adenosine 5'-phosphosulfate sulfotransferase activity. In intact plants, this effect of cyst(e)ine was observed at a concentration of 1 mM, and in plants with excised roots, 0.25 mM had a comparable effect.In developing plants, the extractable activities of O-acetyl-L-serine sulfhydrylase (EC 4.2.99.9) and ribulosebisphosphate carboxylase (EC 4.1.1.39.) were not affected by H2S or cyst(e)ine.
在菜豆初生叶片的叶绿体发育过程中，可提取的腺苷 5′-磷酸硫酸酯硫转移酶的活性增加了十倍。当叶绿体在富含 3.5 μl H2S·l(-1)的空气中发育时，腺苷 5′-磷酸硫酸酯硫转移酶的活性下降。半胱氨酸的浓度高达 1 mM（在外部介质中），不会影响完整植物中腺苷 5′-磷酸硫酸酯硫转移酶活性的增加。在切除根的植物中，0.75 mM 半胱氨酸抑制了这种增加。在绿色初生叶片中，H2S 或半胱氨酸处理导致可提取的腺苷 5′-磷酸硫酸酯硫转移酶活性下降。在完整的植物中，半胱氨酸的这种作用在 1 mM 的浓度下观察到，而在切除根的植物中，0.25 mM 具有类似的作用。在发育中的植物中，H2S 或半胱氨酸对 O-乙酰-L-丝氨酸硫基转移酶（EC 4.2.99.9）和核酮糖二磷酸羧化酶（EC 4.1.1.39.）的可提取活性没有影响。
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