Plantenfysiologisch Laboratorium, Universiteit van Amsterdam, IJdijk 26, Amsterdam, Netherlands.
Planta. 1979 Oct;147(1):51-6. doi: 10.1007/BF00384590.
The relative amounts of Concanavalin A (Con A) bound by gamete and vegetative flagella of both mating types (mt (+) and mt (-)) of Chlamydomonas eugametos were determined using (125)I-Con A. Con A agglutinated all cell types by cross-linking their flagella in a random manner. No correlation was found between the extent of Con A-binding and Con A-mediated isoagglutination. Con A inhibited the sexual interaction between gametes at various levels. In mt (+) gametes it blocked sexual agglutination, whereas in mt (-) gametes it prevented papillar fusion. By SDS-gel electrophoresis nine Con A-binding components were found to be present in flagella. However, it was not possible to allocate a role in sexual agglutination to any of these components since they were present in all cell types, including vegetative cells which are not able to sexually agglutinate.
使用（125）I-Con A 测定了同质接合子（mt(+)和 mt(-)）的配子和营养鞭毛结合的刀豆球蛋白 A（Con A）的相对量。Con A 通过交联它们的鞭毛以随机方式凝集所有细胞类型。Con A 结合的程度与 Con A 介导的同种凝集之间没有相关性。Con A 在不同水平上抑制配子之间的性相互作用。在 mt(+)配子中，它阻止了性凝集，而在 mt(-)配子中，它阻止了纤毛融合。通过 SDS-凝胶电泳，发现鞭毛中有 9 种 Con A 结合成分。然而，由于这些成分存在于所有细胞类型中，包括不能进行性凝集的营养细胞，因此不可能将任何一种成分分配到性凝集中。
