Institut für Allgemeine Botanik der Universität Hamburg, Jungiusstraße 6, D-2000, Hamburg 36, Germany.
Planta. 1979 Dec;147(2):168-73. doi: 10.1007/BF00389520.
Seven day old seedlings of Pisum sativum L., cv. Kleine Rheinländerin, were wilted for 3 days. After partially removing the roots, they were rewatered and at the same time radioactive abscisic acid([1-(14)C]ABA, spec. activity 1.7·10(8)d s(-1)mmol(-1)) was applied for 1 h via the xylem of the roots. After 24 h, 4 days, and 12 days the seedlings were extracted and the metabolites of ABA were analyzed by means of thin-layer and gas chromatography in combination with mass spectrometry, autoradiography, and scintillation counting. Phaseic acid (PA) and dihydrophaseic acid (DPA) were identified as metabolites of ABA. The presence of another ABA-metabolite was also demonstrated. From its mass spectrum it has been postulated that this metabolite is 4'-desoxy-ABA. In addition to these substances, several other metabolites, which are more polar than ABA and its known degradation products, were present in the seedlings. The quantity and number of these unknown metabolites increased with time.
七天大的豌豆（Pisum sativum L.，cv. Kleine Rheinländerin）幼苗萎蔫了三天。部分去除根系后，重新浇水，并同时通过根系的木质部将放射性脱落酸（[1-(14)C]ABA，比活度 1.7·10(8)d s(-1)mmol(-1)）应用 1 小时。24 小时、4 天和 12 天后，幼苗被提取出来，并通过薄层层析和气相色谱结合质谱、放射自显影和闪烁计数分析 ABA 的代谢物。鉴定出黄烷酸（PA）和二氢黄烷酸（DPA）是 ABA 的代谢物。还证明了另一种 ABA 代谢物的存在。根据其质谱，推测该代谢物是 4'-去氧 ABA。除了这些物质外，幼苗中还存在其他几种比 ABA 及其已知降解产物极性更强的代谢物。这些未知代谢物的数量和数量随着时间的推移而增加。
