Lundy E F, Solik B S, Frank R S, Lacy P S, Combs D J, Zelenock G B, D'Alecy L G
J Pharmacol Methods. 1986 Nov;16(3):201-14. doi: 10.1016/0160-5402(86)90042-2.
The Levine rat preparation, the gerbil stroke model, and appropriate control animals were used to determine if the 2,3,5-triphenyltetrazolium chloride (TTC) would selectively identify noninfarcted versus infarcted cerebral tissue. The TTC is frequently used to quantify infarcted myocardial tissue and has been shown to have great specificity, reproducibility, and efficacy. The TTC produces a red product upon reaction with the respiratory enzymes (dehydrogenases) present in non-infarcted tissues. Irreversibly damaged tissues, lacking dehydrogenases, do not form red reaction products. Six gerbil brains and seven rat brains were incubated with the TTC, and the unreacted areas were macroscopically identified. The brains were fixed and sectioned for routine hematoxylin and eosin staining to determine the specificity of the TTC. The TTC was found to react selectively only with non-infarcted cerebral tissue. The gross brain sections were evaluated by macroscopic morphometric analysis, and the unreacted area was always ipsilateral to ligation and correlated with histologic identification of infarct. The brains from neurologically intact animals demonstrated neither macroscopic nor histological evidence of infarction. This technique allows macroscopic quantification of infarct size by planimetry. The average area of infarct for the neurologically impaired rats was 34.7% and it was 31.4% for the impaired gerbils. The percentage of surface area of each infarcted slice was found to correlate with the severity of the neurologic deficit. We conclude that TTC staining is effective for macroscopically delineating cerebral infarcts in rats and gerbils, thus permitting quantification of infarct size.
采用莱文氏大鼠模型、沙鼠中风模型及合适的对照动物，以确定氯化三苯基四氮唑（TTC）能否选择性地鉴别未梗死与梗死的脑组织。TTC常用于定量梗死心肌组织，已证明具有高度特异性、可重复性和有效性。TTC与未梗死组织中存在的呼吸酶（脱氢酶）反应时会产生红色产物。缺乏脱氢酶的不可逆损伤组织不会形成红色反应产物。将6个沙鼠脑和7个大鼠脑用TTC孵育，肉眼识别未反应区域。将脑固定并切片，进行常规苏木精和伊红染色，以确定TTC的特异性。发现TTC仅与未梗死的脑组织选择性反应。通过宏观形态计量分析评估大体脑切片，未反应区域总是与结扎同侧，并与梗死的组织学鉴定相关。神经功能完好动物的脑既无梗死的宏观证据也无组织学证据。该技术可通过平面测量法对梗死面积进行宏观定量。神经功能受损大鼠的平均梗死面积为34.7%，神经功能受损沙鼠为31.4%。发现每个梗死切片的表面积百分比与神经功能缺损的严重程度相关。我们得出结论，TTC染色可有效宏观勾勒大鼠和沙鼠的脑梗死，从而实现梗死面积的定量。
