Department of Analytical Chemistry, University of Córdoba , E-14071 Córdoba, Province of Córdoba, Spain.
Anal Chem. 2014 Jan 7;86(1):783-9. doi: 10.1021/ac403284f. Epub 2013 Dec 17.
In situ synthesis of bare gold nanoparticles mediated by stainless steel as reducing agent was monitored via infrared attenuated total reflection (IR-ATR) spectroscopy. Gold nanoparticles were directly synthesized within the liquid cell of the ATR unit taking immediate advantage of the stainless steel walls of the ATR cell. As nanoparticles were formed, a layer of particles was deposited at the SiO2 ATR waveguide surface. Incidentally, the absorption bands of water increased resulting from surface-enhanced infrared absorption (SEIRA) effects arising from the presence of the gold nanoparticles within the evanescent field. Next to the influence of the Au(III) precursor concentration and the temperature, the suitability of IR-ATR spectroscopy as an innovative tool for investigating changes of nanoparticles in solution, including their aggregation promoted by an increase of the ionic strength or via a pH decrease, and for detailing the sedimentation process of gold nanoparticles was confirmed.
通过红外衰减全反射（IR-ATR）光谱监测了不锈钢介导的裸金纳米粒子的原位合成。金纳米粒子直接在 ATR 单元的液体池中合成，利用 ATR 池的不锈钢壁立即获得优势。随着纳米粒子的形成，一层粒子沉积在 SiO2 ATR 波导表面上。偶然的是，由于金纳米粒子在消逝场中的存在引起的表面增强红外吸收（SEIRA）效应，水的吸收带增加。除了 Au（III）前体浓度和温度的影响之外，IR-ATR 光谱作为一种创新工具，用于研究溶液中纳米粒子的变化，包括通过增加离子强度或通过降低 pH 值促进其聚集，以及详细描述金纳米粒子的沉降过程得到了证实。
