Andani Rafiq, Robertson Ivan, Macdonald Kelli P A, Durrant Simon, Hill Geoffrey R, Khosrotehrani Kiarash
Experimental Dermatology Laboratory, UQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD, Australia.
Exp Dermatol. 2014 Jan;23(1):75-7. doi: 10.1111/exd.12301.
Chronic graft-versus-host disease (cGVHD) is a common complication following allogeneic stem-cell transplantation (SCT). Past studies have implicated the persistence of host antigen-presenting cells (APCs) in GVHD. Our objective was to determine the frequency of host Langerhans cells (LCs) in normal skin post-SCT and ask if their persistence could predict cGVHD. Biopsies of normal skin from 124 sex-mismatched T-cell-replete allogenic SCT recipients were taken 100 days post-transplant. Patients with acute GVHD and those with <9 months of follow-up were excluded and prospective follow-up information was collected from remaining 22 patients. CD1a staining and X and Y chromosome in-situ hybridization were performed to label LCs and to identify their host or donor origin. At 3 months, 59 ± 5% of LCs were host derived. The density of LCs and the proportion of host-derived LCs were similar between patients that did or did not develop cGVHD. Most LCs in the skin remained of host origin 3 months after SCT regardless of cGVHD status. This finding is in line with the redundant role of LCs in acute GVHD initiation uncovered in recent experimental models.
慢性移植物抗宿主病（cGVHD）是异基因干细胞移植（SCT）后的常见并发症。既往研究表明移植物抗宿主病中宿主抗原呈递细胞（APC）持续存在。我们的目的是确定SCT后正常皮肤中宿主朗格汉斯细胞（LC）的频率，并探讨其持续存在是否可预测cGVHD。对124例性别不匹配且T细胞充足的异基因SCT受者在移植后100天进行正常皮肤活检。排除急性移植物抗宿主病患者及随访时间<9个月的患者，并从其余22例患者收集前瞻性随访信息。进行CD1a染色及X和Y染色体原位杂交以标记LC并确定其宿主或供体来源。在3个月时，59±5%的LC来源于宿主。发生或未发生cGVHD的患者之间，LC的密度及宿主来源LC的比例相似。无论cGVHD状态如何，SCT后3个月皮肤中的大多数LC仍来源于宿主。这一发现与近期实验模型中揭示的LC在急性移植物抗宿主病起始中的冗余作用一致。
