State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China.
Virology. 2014 Jan 5;448:322-32. doi: 10.1016/j.virol.2013.10.029. Epub 2013 Nov 9.
Vertebrate interferon (IFN) response defenses against viral infection through the induction of hundreds of IFN-stimulated genes (ISGs). Most ISGs are conserved across vertebrates; however, little is known about the species-specific ISGs. In this study, we reported that grass carp reovirus (GCRV)-induced gene 1 (Gig1), previously screened as a virus-induced gene from UV-inactivated GCRV-infected crucian carp (Carassius auratus) blastulae embryonic (CAB) cells, was a typical fish ISG, which was significantly induced by intracellular poly(I:C) through retinoic acid-inducible gene I (RIG-I)-like receptors-triggered IFN signaling pathway. Transient or stable overexpression of Gig1 prevented GCRV replication efficiently in cultured fish cells. Strikingly, Gig1 homologs were found exclusively in fish species forming a novel gene family. These results illustrate that there exists a Gig1 gene family unique to fish species and the founding gene mediates a novel fish IFN antiviral pathway.
脊椎动物干扰素 (IFN) 通过诱导数百种干扰素刺激基因 (ISGs) 来抵抗病毒感染。大多数 ISGs 在脊椎动物中是保守的；然而，关于种特异性 ISGs 的了解甚少。在这项研究中，我们报道了草鱼虹彩病毒 (GCRV) 诱导基因 1 (Gig1)，它之前作为从紫外线失活的 GCRV 感染的鲫鱼 (Carassius auratus) 囊胚胚胎 (CAB) 细胞中筛选出的病毒诱导基因，是一种典型的鱼类 ISG，通过视黄酸诱导基因 I (RIG-I) 样受体触发的 IFN 信号通路，可被细胞内聚肌胞苷酸 (poly(I:C)) 显著诱导。Gig1 的瞬时或稳定过表达可有效阻止 GCRV 在培养的鱼类细胞中的复制。引人注目的是，Gig1 同源物仅在鱼类物种中发现，形成一个新的基因家族。这些结果表明，存在一个独特的鱼类物种 Gig1 基因家族，该基因家族介导了一种新的鱼类 IFN 抗病毒途径。
