细菌感染（BI）指数：一种改进且简化的快速流式细胞术细菌感染标志物。
Bacterial infection (BI)-INDEX: an improved and simplified rapid flow cytometric bacterial infection marker.
作者信息
Nuutila Jari, Jalava-Karvinen Päivi, Hohenthal Ulla, Kotilainen Pirkko, Pelliniemi Tarja-Terttu, Nikoskelainen Jukka, Lilius Esa-Matti
机构信息
Department of Biochemistry, University of Turku, 20014 Turku, Finland.
Department of Medicine, Turku University Hospital, 20521 Turku, Finland.
出版信息
Diagn Microbiol Infect Dis. 2014 Feb;78(2):116-26. doi: 10.1016/j.diagmicrobio.2013.10.016. Epub 2013 Oct 31.
The aim of this study was to develop a rapid and simple flow cytometric bacterial infection marker. In this prospective comparative study, quantitative flow cytometric analysis of CD10, CD35, CD66b, CD282, and MHC Class I molecules on human neutrophils, monocytes, and B-lymphocytes from 141 hospitalized febrile patients with suspected infection and from 50 healthy controls was performed. We developed a flow cytometric marker of local and systemic bacterial infections, designated "bacterial infection (BI)-INDEX", incorporating the quantitative analysis of CD10, CD35, MHCI, CD66b, and CD282 on neutrophils, monocytes, and B-lymphocytes, which displayed 90% sensitivity and 96% specificity in distinguishing between microbiologically confirmed bacterial (n = 31) and viral infections (n = 27) within a 1-h time-frame. We propose that our novel rapid BI-INDEX test will be useful in assisting physicians to ascertain whether antibiotic treatment is required, thus limiting unnecessary antimicrobial usage.
本研究的目的是开发一种快速简便的流式细胞术细菌感染标志物。在这项前瞻性比较研究中，对141名疑似感染的住院发热患者和50名健康对照者的人类中性粒细胞、单核细胞和B淋巴细胞上的CD10、CD35、CD66b、CD282和MHC I类分子进行了定量流式细胞术分析。我们开发了一种局部和全身细菌感染的流式细胞术标志物，命名为“细菌感染（BI）指数”，它结合了对中性粒细胞、单核细胞和B淋巴细胞上CD10、CD35、MHCI、CD66b和CD282的定量分析，在1小时内区分微生物学确诊的细菌感染（n = 31）和病毒感染（n = 27）时，显示出90%的敏感性和96%的特异性。我们建议，我们新颖的快速BI指数检测将有助于医生确定是否需要抗生素治疗，从而限制不必要的抗菌药物使用。
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