Middleburg Agricultural Research and Extension Center, Middleburg, VA 20117, USA; Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
Domest Anim Endocrinol. 2014 Apr;47:83-91. doi: 10.1016/j.domaniend.2013.11.003. Epub 2013 Nov 20.
The objective of this study was to determine whether the rate of insulin infusion during isoglycemic hyperinsulinemic clamp procedures affected measures of insulin action, including glucose disposal and plasma non-esterified fatty acid, endothelin-1, and nitric oxide concentrations, in mature, healthy horses. Eight thoroughbred mares were studied during a 2-h hyperinsulinemic clamp procedure, conducted at each of 4 rates of insulin infusion: 0 (CON), 1.2 (LOWINS), 3 (MEDINS), and 6 (HIGHINS) mU · kg(-1) · min(-1). The infusion rate of a dextrose solution was adjusted throughout the clamp procedures to maintain blood glucose levels within 10% of baseline glucose concentrations. Plasma insulin concentrations were measured throughout the clamp procedures, and used with the rate of glucose infusion to calculate the plasma insulin concentration-to-rate of glucose infusion ratio, a measure of insulin action on glucose disposal. The rate of glucose infusion increased with rate of insulin infusion (P < 0.05). The plasma insulin concentration-to-rate of glucose infusion ratio was highest for the LOWINS treatment (P < 0.05) and decreased by 62% (P < 0.05) and 84% (P < 0.05) for the MEDINS and HIGHINS treatments, respectively. Although plasma non-esterified fatty acid concentrations were lower than baseline by t = 30 min of the clamp procedures in the LOWINS, MEDINS, and HIGHINS treatments (P < 0.05), the decline was similar for all 3 rates of insulin infusion. Jugular vein plasma nitric oxide and endothelin-1 concentrations were not affected by insulin infusion rate (P > 0.05). The data indicate that it is important to standardize insulin infusion rate if data are to be compared between hyperinsulinemic clamp studies.
本研究旨在确定在等血糖高胰岛素钳夹过程中胰岛素输注率是否会影响胰岛素作用的测量，包括葡萄糖处置以及血浆非酯化脂肪酸、内皮素-1 和一氧化氮浓度，在成熟、健康的马中。在 4 种胰岛素输注率下进行了 2 小时高胰岛素钳夹过程：0（CON）、1.2（LOWINS）、3（MEDINS）和 6（HIGHINS）mU·kg-1·min-1，对 8 匹纯种母马进行了研究。在整个钳夹过程中，通过调整葡萄糖溶液的输注率来维持血糖水平在基础血糖浓度的 10%以内。在整个钳夹过程中测量血浆胰岛素浓度，并与葡萄糖输注率一起计算血浆胰岛素浓度与葡萄糖输注率之比，这是衡量胰岛素对葡萄糖处置作用的指标。葡萄糖输注率随胰岛素输注率的增加而增加（P<0.05）。LOWINS 处理的血浆胰岛素浓度与葡萄糖输注率之比最高（P<0.05），而 MEDINS 和 HIGHINS 处理的分别降低了 62%（P<0.05）和 84%（P<0.05）。尽管在 LOWINS、MEDINS 和 HIGHINS 处理的钳夹过程中，血浆非酯化脂肪酸浓度在 t = 30 分钟时低于基础值（P<0.05），但 3 种胰岛素输注率的下降相似。颈静脉血浆一氧化氮和内皮素-1 浓度不受胰岛素输注率的影响（P>0.05）。数据表明，如果要比较高胰岛素钳夹研究的数据，标准化胰岛素输注率很重要。
