College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing, Jiangsu 210095, China.
Anaerobe. 2014 Feb;25:47-52. doi: 10.1016/j.anaerobe.2013.11.011. Epub 2013 Dec 4.
Equol has higher biological effects than other isoflavones. However, only about 30-50% of humans possess a microbiota capable of producing equol from dietary daidzein. In recent years, interest has grown in dietary applications to improve equol production in human and other animals. In this study, lactulose was used as a potential equol-promoting prebiotic in vitro. The effect of lactulose on transformation of daidzein into equol by sows' fecal microbiota was investigated. Results showed that lactulose treatment improved bacteria growth parameters, changing the kinetics of fermentation in vitro. Lactulose significantly increased total gas production, T1/2, Tmax, and Rmax. Furthermore, lactulose altered the microflora composition, increased equol production associated with a reduction in the population of methanogen and increased the sulfate-reducing bacteria population during 24 h of incubation. Here, we report for the first time that in a certain condition (sealing or high pressure), via a dihydrodaidzein (DHD) pathway equol might be able to reform to daidzein by further metabolism using lactulose as a substrate. This study proposes that "hydrogen-producing prebiotic" might be a novel way to promote equol production in vivo or in vitro.
大豆苷元代谢为黄豆苷元的体外研究。本文研究了乳果糖作为一种潜在的促进黄豆苷元生成黄豆苷元的益生元在体外的效果。结果表明，乳果糖处理可以改善细菌的生长参数，改变发酵的动力学。乳果糖显著增加总气体生成量、T1/2、Tmax 和 Rmax。此外，乳果糖改变了微生物群落组成，增加了与甲烷菌减少相关的黄豆苷元生成量，并增加了硫酸盐还原菌的数量在 24 小时的孵育。在这里，我们首次报道，在一定条件下（密封或高压），通过二氢大豆苷元（DHD）途径，黄豆苷元可能能够通过进一步代谢，以乳果糖为底物重新生成大豆苷元。本研究表明，“产氢益生元”可能是促进体内或体外黄豆苷元生成的一种新方法。
