Hawthorn L, Lan L, Mojica W
Cancer Center, Georgia Regents University, Augusta, GA, USA.
Cancer Center, Georgia Regents University, Augusta, GA, USA.
Genomics. 2014 Feb-Mar;103(2-3):211-21. doi: 10.1016/j.ygeno.2013.11.003. Epub 2013 Dec 4.
We compared transcript expression, and chromosomal changes on a series of tumors and surrounding tissues to determine if there is evidence of field cancerization in colorectal cancer. Epithelial cells were isolated from tumors and areas adjacent to the tumors ranging from 1 to 10cm. Tumor abnormalities mirrored those previously reported for colon cancer and while the number and size of the chromosomal abnormalities were greatly reduced in cells from surrounding regions, many chromosome abnormalities were discernable. Interestingly, these abnormalities were not consistent across the field in the same patient samples suggesting a field of chromosomal instability surrounding the tumor. A mutator phenotype has been proposed to account for this instability which states that the genotypes of cells within a tumor would not be identical, but would share at least a single mutation in any number of genes, or a selection of genes affecting a specific pathway which provide a proliferative advantage.
我们比较了一系列肿瘤及其周围组织的转录本表达和染色体变化，以确定在结直肠癌中是否存在场癌化的证据。从肿瘤及距肿瘤1至10厘米的相邻区域分离出上皮细胞。肿瘤异常情况与先前报道的结肠癌情况相似，虽然周围区域细胞中的染色体异常数量和大小大幅减少，但仍可辨认出许多染色体异常。有趣的是，在同一患者样本中，这些异常在整个区域并不一致，这表明肿瘤周围存在一个染色体不稳定区域。有人提出一种突变体表型来解释这种不稳定性，该表型指出肿瘤内细胞的基因型并非完全相同，但在任意数量的基因中，或在影响特定途径并提供增殖优势的一组基因中，至少会共享一个突变。
